BCSCdb

Number of interactions with A549 is 113

INTERACTOR1	INT1_HGNC_ID	INTERACTOR2	INT2_HGNC_ID	INTERACTION_TYPE	CSC_METHOD	CANCER_TYPE	HISTOLOGICAL_TYPE	CELL_LINE	INTERACTION_METHOD	EXPRESSION_LEVEL	PUBMED_ID
NFATC2	7776	ABCG2	74	Protein-Gene Interaction	FACs for CSC marker	Non Small Cell Lung Cancer	Carcinoma	A549	ChIP-qPCR	Up	33707423
MIR582	32838	BMP4	1071	miRNA-mRNA Interaction	SFA and SP assay	Non Small Cell Lung Cancer	Carcinoma	A549	Transfection with MIR582 mimic followed by qRT-PCR	Up	26468775
MIR582	32838	CCND1	1582	miRNA-mRNA Interaction	SFA and SP assay	Non Small Cell Lung Cancer	Carcinoma	A549	Transfection with MIR582 mimic followed by qRT-PCR	Up	26468775
MIR582	32838	CD44	1681	miRNA-mRNA Interaction	SFA and SP assay	Non Small Cell Lung Cancer	Carcinoma	A549	Transfection with MIR582 mimic followed by qRT-PCR	Up	26468775
ELAVL1	3312	CDK3	1772	RNA-Protein Interaction	FACs followed by SFA	Non Small Cell Lung Cancer	Carcinoma	A549	RIP assay	Up	29344850
MIR582	32838	FGF18	3674	miRNA-mRNA Interaction	SFA and SP assay	Non Small Cell Lung Cancer	Carcinoma	A549	Transfection with MIR582 mimic followed by qRT-PCR	Up	26468775
TBX21	11599	IL4	6014	Protein-Gene Interaction	SP assay and FACs for CSC marker	Non Small Cell Lung Cancer	Carcinoma	A549	ChIP assay followed by PCR	Up	29615105
POU5F1	9221	LGALS3	6563	Gene-Gene Interaction	NA	Non Small Cell Lung Cancer	Carcinoma	A549	Stable forced expression of POU5F1 followed by qRT-PCR	Up	26447186
POU5F1	9221	MMP2	7166	Protein-Gene Interaction	SFA	Non Small Cell Lung Cancer	Carcinoma	A549	ChIP assay followed by Luciferase Reporter assay	Up	24386189
MIR582	32838	MYC	7553	miRNA-mRNA Interaction	SFA and SP assay	Non Small Cell Lung Cancer	Carcinoma	A549	Transfection with MIR582 mimic followed by qRT-PCR	Up	26468775
NFATC2	7776	NOTCH3	7883	Protein-Gene Interaction	FACs for CSC marker	Non Small Cell Lung Cancer	Carcinoma	A549	ChIP-qPCR	Up	33707423
CTNNB1	2514	PROM1	9454	Protein-Gene Interaction	SFA and SP assay	Non Small Cell Lung Cancer	Carcinoma	A549	ChIP assay followed by PCR	Up	26075748
SMARCC2	11105	SNAI2	11094	Protein-Gene Interaction	SFA	Non Small Cell Lung Cancer	Carcinoma	A549	ChIP assay followed by qRT-PCR	Up	27766570
MIR582	32838	TWIST1	12428	miRNA-mRNA Interaction	SFA and SP assay	Non Small Cell Lung Cancer	Carcinoma	A549	Transfection with MIR582 mimic followed by qRT-PCR	Up	26468775
UBE3C	16803	AHNAK	347	Protein-Protein Interaction	SFA	Non Small Cell Lung Cancer	Carcinoma	A549	Co-IP assay followed by Western Blotting	NA	30503554
UBE3C	16803	AHNAK	347	Protein-Protein Interaction	SFA and ALDEFLUOR assay	Non Small Cell Lung Cancer	Carcinoma	A549	Co-IP assay followed by Western Blotting	NA	30503554
CEBPB	1834	ALDH1A1	402	Protein-Gene Interaction	SFA	Non Small Cell Lung Cancer	Carcinoma	A549	ChIP assay followed by qPCR	NA	28420235
RNF168	26661	RHOC	669	Protein-Protein Interaction	NA	Non Small Cell Lung Cancer	Carcinoma	A549	Co-IP assay followed by Western Blotting	NA	34873829
SOX2	11195	CDK1	1722	Protein-Protein Interaction	NA	Non Small Cell Lung Cancer	Carcinoma	A549	Co-IP assay followed by Western Blotting	NA	33721187
CTNNB1	2514	CDH1	1748	Protein-Protein Interaction	SFA	Non Small Cell Lung Cancer	Carcinoma	A549	Co-IP assay followed by Western Blotting	NA	26075748
LGALS3	6563	CTNNB1	2514	Protein-Protein Interaction	SFA followed by FACs	Non Small Cell Lung Cancer	Carcinoma	A549	Co-IP assay followed by Western Blotting	NA	25669973
EFEMP1	3218	EGFR	3236	Protein-Protein Interaction	SFA and ALDEFLUOR assay	Non Small Cell Lung Cancer	Carcinoma	A549	Co-IP assay followed by Western Blotting	NA	24013232
CDK3	1772	ELAVL1	3312	RNA-Protein Interaction	SFA	Non Small Cell Lung Cancer	Carcinoma	A549	RIP assay followed by qRT-PCR	NA	29344850
EFEMP1	3218	IGF1R	5465	Protein-Protein Interaction	SFA	Non Small Cell Lung Cancer	Carcinoma	A549	Co-IP assay followed by Western Blotting	NA	24013232
HSPB1	5246	KLF4	6348	Protein-Protein Interaction	SP assay and SFA	Non Small Cell Lung Cancer	Carcinoma	A549	Co-IP assay followed by Western Blotting	NA	28460458
HSPB1	5246	MYC	7553	Protein-Protein Interaction	SP assay and SFA	Non Small Cell Lung Cancer	Carcinoma	A549	Co-IP assay followed by Western Blotting	NA	28460458
BACH1	935	POU5F1	9221	Protein-Gene Interaction	NA	Non Small Cell Lung Cancer	Carcinoma	A549	ChIP-qPCR	NA	34949193
CD44	1681	POU5F1	9221	Protein-Gene Interaction	NA	Non Small Cell Lung Cancer	Carcinoma	A549	ChIP-qPCR	NA	34949193
HSPB1	5246	POU5F1	9221	Protein-Protein Interaction	SP assay and SFA	Non Small Cell Lung Cancer	Carcinoma	A549	Co-IP assay followed by Western Blotting	NA	28460458
NDRG1	7679	SKP2	10901	Protein-Protein Interaction	SP assay and SFA	Non Small Cell Lung Cancer	Carcinoma	A549	Co-IP assay	NA	28456659
BACH1	935	SOX2	11195	Protein-Gene Interaction	NA	Non Small Cell Lung Cancer	Carcinoma	A549	ChIP-qPCR	NA	34949193
CD44	1681	SOX2	11195	Protein-Gene Interaction	NA	Non Small Cell Lung Cancer	Carcinoma	A549	ChIP-qPCR	NA	34949193
HSPB1	5246	SOX2	11195	Protein-Protein Interaction	SFA	Non Small Cell Lung Cancer	Carcinoma	A549	Co-IP assay followed by Western Blotting	NA	28460458
SNAI2	11094	SOX9	11204	Protein-Protein Interaction	SFA and SP assay	Non Small Cell Lung Cancer	Carcinoma	A549	Co-IP assay followed by Western Blotting	NA	26387547
CTNNB1	2514	SPP1	11255	Protein-Gene Interaction	NA	Non Small Cell Lung Cancer	Carcinoma	A549	ChIP assay followed by RT- PCR	NA	29254164
SOX5	11201	YAP1	16262	Protein-Protein Interaction	SFA	Non Small Cell Lung Cancer	Carcinoma	A549	Co-IP assay followed by Western Blotting	NA	29888108
BACH1	935	NANOG	20857	Protein-Gene Interaction	NA	Non Small Cell Lung Cancer	Carcinoma	A549	ChIP-qPCR	NA	34949193
CD44	1681	NANOG	20857	Protein-Gene Interaction	NA	Non Small Cell Lung Cancer	Carcinoma	A549	ChIP-qPCR	NA	34949193
HSPB1	5246	NANOG	20857	Protein-Protein Interaction	SP assay and SFA	Non Small Cell Lung Cancer	Carcinoma	A549	Co-IP assay followed by Western Blotting	NA	28460458
FENDRR	43894	ABCB1	40	miRNA-mRNA Interaction	SFA	Non Small Cell Lung Cancer	Carcinoma	A549	Luciferase Reporter assay	Down	30981768
CTNNB1	2514	ABCG2	74	Gene-Gene Interaction	NA	Non Small Cell Lung Cancer	Carcinoma	A549	SiRNA mediated knockdown of CTNNB1 followed by qRT-PCR	Down	33980809
SOX2	11195	ABCG2	74	Gene-Gene Interaction	NA	Non Small Cell Lung Cancer	Carcinoma	A549	SiRNA mediated knockdown of SOX2 followed by qRT-PCR	Down	23009336
ATG5	589	ALDH1A1	402	Gene-Gene Interaction	NA	Non Small Cell Lung Cancer	Carcinoma	A549	SiRNA mediated knockdown of ATG5 followed by qRT-PCR	Down	33468994
CTNNB1	2514	ALDH1A1	402	Gene-Gene Interaction	NA	Non Small Cell Lung Cancer	Carcinoma	A549	SiRNA mediated knockdown of CTNNB1 followed by qRT-PCR	Down	33980809
IL6	6018	ALDH1A1	402	Gene-Gene Interaction	NA	Non Small Cell Lung Cancer	Carcinoma	A549	SiRNA mediated knockdown of IL6 followed by qRT-PCR	Down	27009878
ZEB1	11642	ALDH1A1	402	Gene-Gene Interaction	NA	Non Small Cell Lung Cancer	Carcinoma	A549	ShRNA mediated knockdown of ZEB1 followed by qRT-PCR	Down	33468994
FOXD3	3804	ALDH3A1	405	Protein-Gene Interaction	SFA	Non Small Cell Lung Cancer	Carcinoma	A549	ChIP seq followed by qChIp	Down	30703266
TSPYL5	29367	ALDH1A3	409	Gene-Gene Interaction	NA	Non Small Cell Lung Cancer	Carcinoma	A549	SiRNA mediated knockdown of TSPYL5 followed by qRT-PCR	Down	34163000
MIR18A	31548	ATM	795	miRNA-mRNA Interaction	SFA	Non Small Cell Lung Cancer	Carcinoma	A549	Transfection with MIR18A mimic followed by Dual Luciferase Reporter assay	Down	29860718
MIR582	32838	AXIN1	903	miRNA-mRNA Interaction	SFA and SP assay	Non Small Cell Lung Cancer	Carcinoma	A549	Transfection of MIR582 mimics followed by RNA immunoprecipitation assay and qRT-PCR	Down	26468775
NRP1	8004	BMI1	1066	Gene-Gene Interaction	NA	Non Small Cell Lung Cancer	Carcinoma	A549	ShRNA mediated knockdown of NRP1 followed by qRT-PCR	Down	29138851
FOXD3	3804	CBX3	1553	Protein-Gene Interaction	SFA	Non Small Cell Lung Cancer	Carcinoma	A549	ChIP seq followed by qChIp	Down	30703266
BACH1	935	CD44	1681	Gene-Gene Interaction	NA	Non Small Cell Lung Cancer	Carcinoma	A549	ShRNA mediated knockdown of BACH1 followed by qRT-PCR	Down	34949193
TSPYL5	29367	CD44	1681	Gene-Gene Interaction	NA	Non Small Cell Lung Cancer	Carcinoma	A549	SiRNA mediated knockdown of TSPYL5 followed by qRT-PCR	Down	34163000
MIR150	31537	CTNNB1	2514	miRNA-mRNA Interaction	SFA	Non Small Cell Lung Cancer	Carcinoma	A549	Luciferase Reporter assay	Down	31121479
PROM1	9454	CXCR4	2561	Gene-Gene Interaction	NA	Non Small Cell Lung Cancer	Carcinoma	A549	SiRAN mediated knockdown of PROM1 followed by qRT-PCR	Down	28000861
MIR582	32838	DKK3	2893	miRNA-mRNA Interaction	SFA and SP assay	Non Small Cell Lung Cancer	Carcinoma	A549	Transfection of MIR582 mimics followed by RNA immunoprecipitation assay and qRT-PCR	Down	26468775
MIR129-1	31512	DLK1	2907	miRNA-mRNA Interaction	SFA	Non Small Cell Lung Cancer	Carcinoma	A549	Transfection with MIR129-1 mimics followed by qRT-PCR and Western Blotting	Down	28619508
MIR708	33654	DNMT3A	2978	miRNA-mRNA Interaction	SFA	Non Small Cell Lung Cancer	Carcinoma	A549	Transfection with MIR708 mimics followed by Luciferase Reporter assay and Western Blotting	Down	28972040
MIR1290	35283	FRK	3955	miRNA-mRNA Interaction	SFA	Non Small Cell Lung Cancer	Carcinoma	A549	Transfection with miR1290 mimmics followed by Luciferase Reporter assay	Down	25783528
MIR410	32065	GSK3B	4617	miRNA-mRNA Interaction	SFA	Non Small Cell Lung Cancer	Carcinoma	A549	Transfection of MIR410 mimics followed by Luciferase Reporter assay	Down	28076327
MIR18A	31548	HIF1A	4910	miRNA-mRNA Interaction	SFA	Non Small Cell Lung Cancer	Carcinoma	A549	Transfection with MIR18A mimic followed by Dual Luciferase Reporter assay	Down	29860718
FOXD3	3804	HMGB1	4983	Protein-Gene Interaction	SFA	Non Small Cell Lung Cancer	Carcinoma	A549	ChIP seq followed by qChIp	Down	30703266
MIR150	31537	HMGA2	5009	miRNA-mRNA Interaction	SFA	Non Small Cell Lung Cancer	Carcinoma	A549	Luciferase Reporter assay	Down	31121479
POU5F1	9221	MMP2	7166	Gene-Gene Interaction	SFA	Non Small Cell Lung Cancer	Carcinoma	A549	ShRNA mediated knockdown of POU5F1 followed by qRT-PCR	Down	24386189
MIR128-1	31510	MUC1	7508	miRNA-mRNA Interaction	SFA	Non Small Cell Lung Cancer	Carcinoma	A549	Transfection with MIR128-1 followed by Luciferase Reporter assay	Down	29299167
HDAC3	4854	MYC	7553	Protein-Gene Interaction	SFA	Non Small Cell Lung Cancer	Carcinoma	A549	ChIP assay followed by PCR	Down	30258097
ITGB1	6153	MYC	7553	Gene-Gene Interaction	NA	Non Small Cell Lung Cancer	Carcinoma	A549	ShRNA mediated knockdown of ITGB1 followed by RT-PCR	Down	30485693
SLC16A1	10922	MYC	7553	Gene-Gene Interaction	NA	Non Small Cell Lung Cancer	Carcinoma	A549	ShRNA mediated knockdown of ZIC2 followed by qRT-PCR	Down	32922547
SOX2	11195	MYC	7553	Gene-Gene Interaction	NA	Non Small Cell Lung Cancer	Carcinoma	A549	ShRNA mediated knockdown of SOX2 followed by RT-PCR	Down	22615765
TP53	11998	MYC	7553	Protein-Gene Interaction	SFA	Non Small Cell Lung Cancer	Carcinoma	A549	ChIP assay followed by qRT-PCR	Down	30503554
UBE3C	16803	MYC	7553	Gene-Gene Interaction	NA	Non Small Cell Lung Cancer	Carcinoma	A549	ShRNA mediated knockdown of UBE3C followed by qRT-PCR	Down	30503554
ZNF322	23640	MYC	7553	Protein-Gene Interaction	SFA	Non Small Cell Lung Cancer	Carcinoma	A549	ChIP assay followed by PCR	Down	30258097
SOX2	11195	NOTCH1	7881	Gene-Gene Interaction	NA	Non Small Cell Lung Cancer	Carcinoma	A549	ShRNA mediated knockdown of SOX2 followed by RT-PCR	Down	22615765
ATG5	589	POU5F1	9221	Gene-Gene Interaction	NA	Non Small Cell Lung Cancer	Carcinoma	A549	SiRNA mediated knockdown of ATG5 followed by qRT-PCR	Down	33468994
BACH1	935	POU5F1	9221	Gene-Gene Interaction	NA	Non Small Cell Lung Cancer	Carcinoma	A549	ShRNA mediated knockdown of BACH1 followed by qRT-PCR	Down	34949193
IL6	6018	POU5F1	9221	Gene-Gene Interaction	NA	Non Small Cell Lung Cancer	Carcinoma	A549	SiRNA mediated knockdown of IL6 followed by qRT-PCR	Down	27009878
ITGB1	6153	POU5F1	9221	Gene-Gene Interaction	NA	Non Small Cell Lung Cancer	Carcinoma	A549	ShRNA mediated knockdown of ITGB1 followed by RT-PCR	Down	30485693
LDHA	6535	POU5F1	9221	Gene-Gene Interaction	NA	Non Small Cell Lung Cancer	Carcinoma	A549	ShRNA mediated knockdown of LDHA followed by qRT-PCR	Down	34178639
NRP1	8004	POU5F1	9221	Gene-Gene Interaction	NA	Non Small Cell Lung Cancer	Carcinoma	A549	ShRNA mediated knockdown of NRP1 followed by qRT-PCR	Down	29138851
TCF7	11639	POU5F1	9221	Gene-Gene Interaction	NA	Non Small Cell Lung Cancer	Carcinoma	A549	ShRNA mediated knockdown of lnc TCF 7 followed by qRT-PCR	Down	27766570
ZEB1	11642	POU5F1	9221	Gene-Gene Interaction	NA	Non Small Cell Lung Cancer	Carcinoma	A549	ShRNA mediated knockdown of ZEB1 followed by qRT-PCR	Down	33468994
UBE3C	16803	POU5F1	9221	Gene-Gene Interaction	NA	Non Small Cell Lung Cancer	Carcinoma	A549	ShRNA mediated knockdown of UBE3C followed by qRT-PCR	Down	30503554
MIR122	31501	PRDX2	9353	miRNA-mRNA Interaction	Enrichment by Gefitinib drug	Non Small Cell Lung Cancer	Carcinoma	A549	Transfection with MIR122 mimic followed by Western Blotting and ICC staining	Down	30341415
ATG5	589	PROM1	9454	Gene-Gene Interaction	NA	Non Small Cell Lung Cancer	Carcinoma	A549	SiRNA mediated knockdown of ATG5 followed by qRT-PCR	Down	33468994
IL6	6018	PROM1	9454	Gene-Gene Interaction	NA	Non Small Cell Lung Cancer	Carcinoma	A549	SiRNA mediated knockdown of IL6 followed by qRT-PCR	Down	27009878
ITGB1	6153	PROM1	9454	Gene-Gene Interaction	NA	Non Small Cell Lung Cancer	Carcinoma	A549	ShRNA mediated knockdown of ITGB1 followed by RT-PCR	Down	30485693
SLC16A1	10922	PROM1	9454	Gene-Gene Interaction	NA	Non Small Cell Lung Cancer	Carcinoma	A549	Lentiviral mediated knockdown of SLC16A1 followed by qRT-PCR	Down	31891569
ZEB1	11642	PROM1	9454	Gene-Gene Interaction	NA	Non Small Cell Lung Cancer	Carcinoma	A549	ShRNA mediated knockdown of ZEB1 followed by qRT-PCR	Down	33468994
MIR5100	43540	RAB6A	9786	miRNA-mRNA Interaction	SFA	Non Small Cell Lung Cancer	Carcinoma	A549	Transfection with MIR5100 mimic followed by Luciferase Reporter assayand Western Blotting	Down	29144562
MIR485	32067	RXRA	10477	miRNA-mRNA Interaction	SFA	Non Small Cell Lung Cancer	Carcinoma	A549	Transfection with MIR485 mimic followed by Dual Luciferase Reporter assay	Down	30058740
FOXD3	3804	SET	10760	Protein-Gene Interaction	SFA	Non Small Cell Lung Cancer	Carcinoma	A549	ChIP seq followed by qChIp	Down	30703266
MIR582	32838	SFRP1	10776	miRNA-mRNA Interaction	SFA and SP assay	Non Small Cell Lung Cancer	Carcinoma	A549	Transfection of MIR582 mimics followed by RNA immunoprecipitation assay and qRT-PCR	Down	26468775
BACH1	935	SOX2	11195	Gene-Gene Interaction	NA	Non Small Cell Lung Cancer	Carcinoma	A549	ShRNA mediated knockdown of BACH1 followed by qRT-PCR	Down	34949193
IL6	6018	SOX2	11195	Gene-Gene Interaction	NA	Non Small Cell Lung Cancer	Carcinoma	A549	SiRNA mediated knockdown of IL6 followed by qRT-PCR	Down	27009878
ITGB1	6153	SOX2	11195	Gene-Gene Interaction	NA	Non Small Cell Lung Cancer	Carcinoma	A549	ShRNA mediated knockdown of ITGB1 followed by RT-PCR	Down	30485693
LDHA	6535	SOX2	11195	Gene-Gene Interaction	NA	Non Small Cell Lung Cancer	Carcinoma	A549	ShRNA mediated knockdown of LDHA followed by qRT-PCR	Down	34178639
TCF7	11639	SOX2	11195	Gene-Gene Interaction	NA	Non Small Cell Lung Cancer	Carcinoma	A549	ShRNA mediated knockdown of lnc TCF 7 followed by qRT-PCR	Down	27766570
TP53	11998	SOX2	11195	Protein-Gene Interaction	SFA	Non Small Cell Lung Cancer	Carcinoma	A549	ChIP assay followed by qRT-PCR	Down	30503554
TCF7	11639	EpCAM	11529	Gene-Gene Interaction	NA	Non Small Cell Lung Cancer	Carcinoma	A549	ShRNA mediated knockdown of lnc TCF 7 followed by qRT-PCR	Down	27766570
FOXD3	3804	WDR5	12757	Protein-Gene Interaction	SFA	Non Small Cell Lung Cancer	Carcinoma	A549	ChIP seq followed by qChIp	Down	30703266
FOXD3	3804	NSD2	12766	Protein-Gene Interaction	SFA	Non Small Cell Lung Cancer	Carcinoma	A549	ChIP seq followed by qChIp	Down	30703266
SOX2	11195	WNT1	12774	Gene-Gene Interaction	NA	Non Small Cell Lung Cancer	Carcinoma	A549	ShRNA mediated knockdown of SOX2 followed by RT-PCR	Down	22615765
MIR214	31591	CTNNBIP1	16913	miRNA-mRNA Interaction	SFA	Non Small Cell Lung Cancer	Carcinoma	A549	Transfection with MIR214 mimmics followed by Luciferase Reporter assay and qRT-PCR	Down	26299367
MIR150	31537	GSKIP	20343	miRNA-mRNA Interaction	SFA	Non Small Cell Lung Cancer	Carcinoma	A549	Luciferase Reporter assay	Down	31121479
ATG5	589	NANOG	20857	Gene-Gene Interaction	NA	Non Small Cell Lung Cancer	Carcinoma	A549	SiRNA mediated knockdown of ATG5 followed by qRT-PCR	Down	33468994
BACH1	935	NANOG	20857	Gene-Gene Interaction	NA	Non Small Cell Lung Cancer	Carcinoma	A549	ShRNA mediated knockdown of BACH1 followed by qRT-PCR	Down	34949193
CTNNB1	2514	NANOG	20857	Gene-Gene Interaction	NA	Non Small Cell Lung Cancer	Carcinoma	A549	SiRNA mediated knockdown of CTNNB1 followed by qRT-PCR	Down	33980809
IL6	6018	NANOG	20857	Gene-Gene Interaction	NA	Non Small Cell Lung Cancer	Carcinoma	A549	SiRNA mediated knockdown of IL6 followed by qRT-PCR	Down	27009878
ITGB1	6153	NANOG	20857	Gene-Gene Interaction	NA	Non Small Cell Lung Cancer	Carcinoma	A549	ShRNA mediated knockdown of ITGB1 followed by RT-PCR	Down	30485693
LDHA	6535	NANOG	20857	Gene-Gene Interaction	NA	Non Small Cell Lung Cancer	Carcinoma	A549	ShRNA mediated knockdown of LDHA followed by qRT-PCR	Down	34178639
TCF7	11639	NANOG	20857	Gene-Gene Interaction	NA	Non Small Cell Lung Cancer	Carcinoma	A549	ShRNA mediated knockdown of lnc TCF 7 followed by qRT-PCR	Down	27766570
ZEB1	11642	NANOG	20857	Gene-Gene Interaction	NA	Non Small Cell Lung Cancer	Carcinoma	A549	ShRNA mediated knockdown of ZEB1 followed by qRT-PCR	Down	33468994