LncRbase v2 other
About lncRNA subtypes and ID assignment

1. lncRNA subtypes

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LncRBase hosts seventeen distinct subtypes of lncRNAs including our newly classified types and other transcript biotypes.
These are as follows: 
(1) 3UTR, 3/UTR overlapping lncRNAs overlapping any 3/UTR exon in the sense strand. 
(2) 5UTR, 5/UTR overlapping lncRNAs overlapping any 5/UTR exon in the sense strand lncRNA 
(3) CDS, CDS overlapping lncRNAs overlapping any protein coding CDS exon. 
(4) LI, Intergenic (linc) lncRNAs transcribed from in between two gene loci 
(5) AO, Antisense lncRNAs intersecting any exon of a protein-coding locus on the opposite strand 
(6) CI, Completely Intronic transcripts reside within introns of a coding gene, but do not intersect any exons. 
(7) AI, Intronic Antisense lncRNAs that completely overlap with an intron in the opposite strand 
(8) IO, Intron Overlapping lncRNA splice variants of a gene, contain intronic sequence 
(9) PS, Pseudogene transcripts having homology to protein coding transcripts but containing disrupted coding sequence. 
(10) SO, Sense Overlapping lncRNAs containing a coding gene in its intron on the same strand 
(11) AG, Ambiguous transcripts cannot be confined within a single biotype category, falling under two or more biotypes.
(12) PT, Processed Transcript not containing an ORF and cannot be placed in any of the above categories 
(13) MI, miscRNA from the Ensembl transcript dataset. 
(14) NC, Non coding transcripts not falling in any of the above mentioned categories.
(15) Mito_M, lncRNA transcripts originating from chromosome M.
(16) Macro, Unspliced lncRNAs that are several kb in size.
(17) BI, Bidirectional promoter lncRNA.

2. Assignment of lncRNA IDs

Based on the chromosomal location, we assigned unique identifiers to each transcript. Human lncRNA transcript identifiers (ID) begin with hsaLB. Human lncRNA transcripts have been designated a unique identifier as hsaLB_[type]_[numeral]. 'type' is the distinct subtype of the lncRNA transcript IDs. The numeral ({primary}.{secondary}) is assigned as per the chromosomal location of the transcript. Multiple transcripts from the same genomic locus have same {primary} number with increasing {secondary} number which represents different cDNA variants from the locus. Similar naming convention has been followed for mouse, drosophila, zebrafish, cow, chicken, rat, c.elegans lncRNA transcripts, preceded by 'mmu', ‘dre’, ‘dme’, ‘bta’, ‘gga’, ‘rno’, and ‘cel’ prefix respectively.

 

Search LncRBase v.2

 

1. LncRNA transcripts

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5 search options are available in LncRBase v2 under Section Transcripts.
1. Search by Accession ID. First select the Organism name, then type a. LncRBase v2 ID to view detailed information on that transcript. or b. Original ID (Ensembl/Noncode v5.0/UCSC Gene ID/NCBI Refseq /H-InvDB 9.0). Clicking Submit displays detailed information about that lncRNA.
2. Search by Gene Symbol. To search for a specific lncRNA, first, select the Organism name, then type its gene name or gene symbol (HGNC/MGI/ZFIN/RGD/FlyBase/WormBase). You will be shown the number of entries for that gene in LncRBase and associated literature. Clicking on the particlar gene loci will show you the details of the gene and correponding transcript variants that are hosted in LncRBase.
3. Browse by lncRNA subtype, coding potential and sORF overlap. In the first segment, select organism first. Then select any specific lncRNA biotype, chromosomal position, coding potential tool name and coding potential type to view the corresponding information. For the second segment, organism and chromosome input would retrieve the sORF count of overlapped lncRNAs.
4. Search for associated genomic elements. First, select the organism. Then select either th Repeat/ CGI/ Microarray probes option and chromosome no. to view Repeat overlap, CGI in lncRNA promoters and remapped Affymetrix Microarray Probes respectively.
5. Browse small ncRNA associated lncRNAs. Firstly, select the organism. Then select either the miRNA or piRNA option and chromosome no. to view lncRNA-associated miRNA or piRNAs.

2. LncRNA expression

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Click any of the eight (8) organism name under section “Expression” to open the organism-specific expression query page. Select a specific “tissue” to show all the corresponding data “accession IDs”. You can further filter your query by selecting type of lncRNA you want to search. In the output page you will be shown the total number of transcripts in that biotype and their average FPKM value in the selected tissue.

 

View results of your search

 

1. Detail Output of LncRBase ID Search

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Clicking on an individual transcript ID in the general output page will direct you to a detailed output page. You can view LncRBase ID, Original ID, Alias ID , Gene name, genomic location, transcript length, %GC, Coding Potential score and predicted localization. To view detailed information on sORF count , associated miRNAs, associated piRNAs, number of microarray probes mapped to that lncRNA, predicted localization, associated Repeat Element, CGI, target genes, disease association and TFBS in lncRNA promoter well as lncRNA expression of various tissues, click on the view button.

2. ID Conversion

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Select the organism( only for human and mouse) and input v2 ID to retrieve v1 ID if present. Click on the link to go to LncRBase v1 webpage of transcription ID details.

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